headspace strain genetics

Relative to the reference strain (C57BL/6J), CAST/EiJ has lost 20 olfactory receptors and gained 37 gene family members: 12 novel and 25 supported by published predictions based on messenger RNA (mRNA) derived from CAST/EiJ whole olfactory mucosa (Fig. Strains Calm. Get what matters in translational research, free to your inbox weekly. The primer binding site in PWK/PhJ is the same as that in C57BL/6J, however Nlrp1c is a predicted pseudogene. Headspace Weed Strain Information | Leafly From the pure landraces up Natl Acad. 84, 81728180 (2010). Would you like email updates of new search results? 1d). Strain-specific gene sets (Supplementary Data 3) and PantherDB classifications are contained in Supplementary Table 10. For the remaining reads (approximately 70%), strain-specific exonpart and intron hints were generated. 41, D377D386 (2013). We're still trying figure out what flavors or effects you've experienced - if it was good leave us an review below so other users know too Putnam, N. H. et al. PLoS Genet. 9, 10991107 (2007). However, the most consistent reports maintain that a Cherry Pie/Burmese Thai cross makes official website and that any information you provide is encrypted Pairs of flanking guide RNAs (gRNAs) were designed using the WTSI Genome Editing (WGE) tool78 creating four gRNAs (two gRNAs 5 and two gRNAs 3 to the CE region, Supplementary Table 21). An improved approach to mate-paired library preparation for Illumina sequencing. THC (tetrahydrocannabinol) is the primary psychoactive compound in cannabis and is responsible for producing the "high" associated with marijuana use. Schrider, D. R. et al. Cosubstrate independent mineralization of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a Desulfovibrio species under anaerobic conditions. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Interestingly, the previously unannotated Efcab3-like gene is very close to the 17q21.31 syntenic region associated in humans to the Koolende Vries microdeletion syndrome (KdVS). Expression of H60 on mice heart graft and influence of cyclosporine. Epub 2018 Nov 28. Ye, Z. et al. BMC Genomics 14, 188 (2013). Many novel genes and transcripts were identified across all of the strains, highlighting unexplored sequence variation across the Mus lineage. Yalcin, B. et al. a, Summary of the strain-specific gene sets showing the number of genes broken down by GENCODE biotype. Simpson, E. M. et al. Each strain has a unique haplotype of Nlrp1 members, highlighting the extensive sequence diversity at this locus across inbred mouse strains (Fig. Brain sections were double-stained using luxol fast blue for myelin and cresyl violet for neurons and scanned at cell-level resolution using the Nanozoomer whole-slide scanner (Hamamatsu Photonics). Doran, A. G. et al. In AugustusCGP the same types of evidence can be incorporated for either a subset or all species/ strains. 9). Sour Diesel BX2 (aka Sowahh) byApeOrigin - hergestellt mit offener Bestubung der Sowahh von Karma. Nat. Patierno, S. R. et al. 7, 118130 (2007). Proc. Monroe, K. M. et al. WebAlthough its exact genetics and breeder arent documented, this bud may have a connection to an Afghani indica landrace. It helps stimulate an appetite in people who have trouble eating due to stress or depression. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Large gene families in which little functional information is known were also identified. In the de novo assemblies, both mouse strains share the same promoter region for Nlrp1c; however, when transcribed, the cDNA of Nlrp1c_CAST could not be amplified with previously designed primers54 due to SNPs at the primer binding site (5CACT-3 5TACC-3). Stanke, M., Diekhans, M., Baertsch, R. & Haussler, D. Using native and syntenically mapped cDNA alignments to improve de novo gene finding. David Downs. J. Immunol. Discover 7 surprising ways to use hemp in your daily life, from beauty products to food. Taylor, G. A. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Lilue, J., Mller, U. Check it out and see what will happen if you click onto the Hybrids! Biol. d, Representative sagittal brain images of matched controls (left) and Efcab3-like mutant (right), showing a larger cerebellum, enlarged lateral ventricle and increased size of the pontine nuclei (n=3, see Supplementary Fig. WebAfter cultivation with 21% (v/v) O2 in the headspace, no recombinant hydrogenase activity was observed in soluble extracts of wild-type BL21, although low levels of activity could be Some of the most potent terpenes are: In terms of smell and taste, it's pretty much exactly what you'd expect if you're familiar with the classic Headspace taste. Immunol. We'll explore top-quality strains, knowledgeable budtenders, and more. This strain has also been speculated as Bob Marleys favorite. Unauthorized use of these marks is strictly prohibited. A digital atlas of gene expression patterns in the mouse: http://www.genepaint.org, A pipeline used to comparatively annotate the mouse strains for the Mouse Genomes Project: https://github.com/ucsc-mus-strain-cactus/MouseGenomesAnnotationPipeline, SGA String Graph Assembler a de novo genome assembler: https://github.com/jts/sga, SNAP Scalable Nucleotide Alignment Program a new sequence aligner: http://snap.cs.berkeley.edu, ImageJ an image processing toolkit: https://imagej.nih.gov/ij/. Newly annotated members Nlrp1b2 and Nlrp1d appear functionally intact in CAST/EiJ but were both predicted as pseudogenes in PWK/PhJ due to the presence of stop codons or frameshift mutations. Clipboard, Search History, and several other advanced features are temporarily unavailable. Strain Headspace is a relatively new hybrid marijuana strain. & Howard, J. C. The immunity-related GTPases in mammals: a fast-evolving cell-autonomous resistance system against intracellular pathogens. Nucleic Acids Res. is supported by R01 DK074656. Manual annotation extended this gene to 188 exons (lower red track). 38, 21682171 (2006). Parasitology 142(Suppl, 1), S108S119 (2015). Pseudochromosomes were produced in parallel utilizing cross-species synteny alignments resulting in genome assemblies of between 2.254 (WSB/EiJ) and 2.328 gigabases (Gb) (AKR/J) excluding unknown gap bases. Levinsohn, J. L. et al. These regions are often of variable copy number between various haplotypes69. Nat. 1, https://doi.org/10.2478/mngs-2013-0001 (2013). 306, L10L22 (2014). Box plots show 25th and 75th percentiles, and the median value. In humans, SLFN11 was reported to inhibit HIV protein synthesis by a codon-usage-based mechanism57 and in non-human primates positive selection on the gene Slfn11 has been reported58. We found an 18 amino acid mismatch in the nucleotide-binding domain (NBD) between Nlrp1b_CAST and Nlrp1b_PWK. With its high-THC content, Train Wreck can relieve various mood and health-related conditions, making it a go-to for people needing medicinal relief. Cannabinoids are chemical compounds found in cannabis that interact with the body's endocannabinoid system to produce various effects. Biotin-containing fragments were then isolated using streptavidin beads before PCR enrichment of each library. Both CAST/EiJ and PWK/PhJ contain functional copies of Sfln10, which is a predicted pseudogene in C57BL/6J and WSB/EiJ. Beck, J. It's a delicious way to enjoy the benefits of cannabis in a cool treat. Liu, Q. et al. Cannabis 21, 12391248 (2011). The Headspace strain is hybrid cannabis that has a THC-rich cannabinoid profile with a THC content of 18-19%, while CBD content ranges between 0.55% and 0.82%. These classifiers include evaluating if a transcript maps multiple times, the proportion of unknown bases, splice site validity, both frameshifting and non-frameshifting indels and small alignment gaps. It's also good for growing outdoors because it has an average flowering time of 54-61 days, and it can reach heights of 60-80 cm (24 - 32 inches). WebSeedFinders Cannabis-Genetic-Search Origin and genetic characteristics Here you can look for Cannabis Strains who have certain genetic characteristics. Approximately 0.52% of total genome length per strain was unplaced and is composed of unknown gap bases (1849%) and repeat sequences (6179%) (Supplementary Table 2), with between 89 and 410 predicted genes per strain (Supplementary Table 3). It takes well to most growing mediums too, including Wild-derived strains have been crossed with classical strains to create powerful resources such as the Collaborative Cross (CC) and Diversity Outbred Cross (DO) in which genetic traits have been mapped7,8,9,10. Mol. Nature 409, 860921 (2001). Strains For Female Arousal b, Evolutionary history of Efcab3-like in vertebrates including genome structure and surrounding genes. Frosted Cherry Cookies is a great daytime strain for experienced users, but also pleasant for anyone smoking at the end of the night. 2a and Supplementary Table 13)42. a, Olfactory receptor genes on chromosome 11 of CAST/EiJ. WebIts easy to grow even if this is your first-ever strain. WebIn live cultures containing an N2-CO2 headspace, RDX was not degraded, and no acetate was formed. 1). 35, 222236 (2015). Ragout classified 38% of them as valid rearrangements and the rest as misassemblies (which were removed). Broz, P. & Dixit, V. M. Inflammasomes: mechanism of assembly, regulation and signalling. Thus, their identification is a powerful tool for finding errors in genome assemblies. This strain has become popular for its libido-boosting effects that will put you in the mood for romance. Gene prediction based on PacBio cDNA sequencing introduced an average of 1,865 further new isoforms to CAST/EiJ, PWK/PhJ, and SPRET/EiJ. The 16S rRNA gene sequence for strain HAAP-1, consisting of 1485 base pairs, Headspace Cannabis Strain - Discover Its Effects, Genetics, and Collaborative Cross mice and their power to map host susceptibility to Aspergillus fumigatus infection. Explore the rich history of cannabis, from its earliest recorded use to its current place in society. Nat. Gene retrotransposition has long been implicated in the creation of gene family diversity36, novel alleles conferring positively selected adaptations37. Finally, alignment of PacBio long-read complementary DNA sequences from liver and spleen of C57BL/6J, CAST/EiJ, PWK/PhJ, and SPRET/EiJ showed that the GRCm38 reference genome had the highest proportion of correctly aligned cDNA reads (99% and 98%, respectively) and the strains and MGSCv3 were 12% lower (Supplementary Table 7). The origin of the strain is very controversial, but, in both instances, its genealogy includes OG Kush and Durban Poison, which gives it a minty and pungent punch with a sweet after-burst. In live cultures containing an N2-CO2 headspace, RDX was not degraded, and no acetate was formed. Proc. 38, 240244 (2006). Nat. Sastalla, I. et al. A recombination event happened in the common ancestor of subfamily Homininae, which disrupted Efcab3-like in gorilla (G. gorilla) and human (H. sapiens). doi: 10.1002/mbo3.423. 16, 1927 (1997). Genome Res. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Learn about safe consumption, the impact of legalization, and how you can join the festivities! For example, 23 distinct clusters of olfactory receptors were identified, indicating substantial variation among inbred strains. As a result, the total brain area parameter was enlarged by 7% (P=0.006). Modeling host genetic regulation of influenza pathogenesis in the collaborative cross. Positive F0 mice were further bred with C57BL/6NTac mice. First off, the smell is earthy and pungent like a forest after rainfall. Genes involved in defense and immunity (the largest protein class represented by the combined gene set) were then retrieved and the strains that contributed genes to this protein class identified. Previous variation catalogs have indicated high concordance (>97% shared SNPs) between NZO/HlLtJ and another inbred laboratory strain NZB/BlNJ21. However, we are left with six loci (57 kb) enriched for hSNPs in C57BL/6J and C57BL/6NJ that do not have an obvious explanation and could be attributed to residual heterozygosity. For every strain into our database you will be able to create an unique map for all known hybrids and crossings! These initial orthologs, along with strain-specific RNA-Seq (Supplementary Table 8), were input to AUGUSTUS74 one at a time to apply local strain-specific refinement. Seq. Lilue, J., Doran, A.G., Fiddes, I.T. Genome Res. RNA-Seq splicing from two tissues (B=Brain, L=Liver, blue tracks) and five strains are displayed. The research leading to these results has received funding from the European Unions Seventh Framework Programme (FP7/20072013) under grant agreement HEALTH-F4-2010-241504 (EURATRANS). WebCannabis sativa (cannabis or marijuana) contains over 100 different terpenes and terpenoids, including mono, sesqui, di and tri, as well as other miscellaneous compounds of terpenoid orgin. In the meantime, to ensure continued support, we are displaying the site without styles Stremlau, M. et al. The 16S rRNA gene sequence for strain HAAP-1, consisting of 1485 base pairs, had a 99.2% and 99.1% sequence similarity to Acetobacterium malicum and A. wieringae, respectively. Before 3a). Epub 2016 Nov 17. We are here to help! All rights reserved. Skarnes, W. C. et al. Libido definitely improved. The genome assemblies and annotation are available via the Ensembl genome browser and the UCSC Genome Browser. The most well-known cannabinoids are THC (tetrahydrocannabinol), which is responsible for the psychoactive effects of cannabis, and CBD (cannabidiol), which is non-intoxicating and known for its potential therapeutic properties. Zhang, H., Hardamon, C., Sagoe, B., Ngolab, J. Terpenes are aromatic compounds found in cannabis and other plants that contribute to each strain's unique scent and flavor profile. The latest in Strains & products. Biodegradation of the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in cold marine sediment under anaerobic and oligotrophic conditions. In particular, the wild-derived strains represent a rich resource of novel target sites, resistance alleles, genes and isoforms not present in the reference strain, or indeed many classical strains. Mari profitability report. 1, 119126 (2000). The sensory results Top autoflower seeds to grow for spring and summer 2023. Medical marijuana patients choose Headspace to help relieve symptoms of stress, pain and anxiety. 3a) and an Efcab3-like knockout, showed analogous brain phenotypes, suggesting common cis-acting regulatory mechanisms as shown previously in the context of the 16p11.2 microdeletion syndrome68. Nature 491, 125128 (2012). Bubblegum Popperz by Superdope, HiTech and Fear of Boof The Nlrp1 locus (NOD-like receptors, pyrin domain-containing) encodes inflammasome components that sense endogenous microbial products and metabolic stresses, thereby stimulating innate immune responses51. Nature 477, 326329 (2011). A conditional knockout resource for the genome-wide study of mouse gene function. We're still trying to learn more about the flavors and effects of Headspace - if you've smoked, dabbed, or consumed this strain before, tell us about it by leaving a review. 21, 15121528 (2011). The yield indoors depends on the growing area and can be as much as 1 ounce per square foot. Consult with a cannabis healthcare professional for specific medical concerns, and always verify the legality of cannabis in your location. WebBred by Liberty Reach, this strain is tasty like the classic GSC with a sweet profile thats also crisp and fresh. Each of the genomes was assembled into a complete set of chromosomes with less than 5% of unlocalized sequence (Supplementary Data 10). OG Kush The Strains aroma is woodsy or piney, and pungent is the best way to describe it. Correspondence to We discovered a novel 188-exon gene on chromosome 11 that significantly extends the existing gene Efcab3 spanning between Itgb3 and Mettl2 (Fig. Nature 456, 724727 (2008). Rev. e, Box plot of sequence divergence (%)for LTRs, LINEs, and SINEs within and outside of hSNP regions. We have completed the first draft de novo assemblies and strain-specific gene annotation for 12 classical inbred laboratory mouse strains (129S1/SvImJ, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NZO/HlLtJ, and NOD/ShiLtJ) and 4 wild-derived strains representing the backgrounds Mus musculus castaneus (CAST/EiJ), M. m. musculus (PWK/PhJ), M. m. domesticus (WSB/EiJ), and M. spretus (SPRET/EiJ). The scaffolds were assembled into chromosome-scale scaffolds using Ragout v2.0. Mol. Accessibility 8600 Rockville Pike Taken together, these results suggest a potential role of the Efcab3-like gene to regulate brain development and brain size from the forebrain to the hindbrain. 2b and Supplementary Figs. Combinations of alleles and genes unique to an individual strain are commonly observed at these loci, reflecting distinct strain phenotypes. Neuroanatomical studies were performed blind with experimenters not knowing the genotype of the mouse, on three 16-week-old matched control male mice in C57BL/6N background and three 16-week-old homozygous knockout of Efcab3. Each of the three M. m. domesticus strains (C67BL/6J, NOD/ShiLtJ, and WSB/EiJ) carries a different combination of Nlrp1 family members; Nlrp1d1f are novel strain-specific alleles that were previously unknown. 2d). Cannabis Genetic Search :: Origin and genetic Second, C57BL/6J reads aligned to the regions of interest in the C57BL/6NJ assembly were extracted for targeted assembly, leading to the generation of contigs covering sequences currently missing from the reference. Consider factors such as the type of experience you're seeking (e.g., relaxation, pain relief, creativity), your tolerance level, and any medical conditions or symptoms you wish to address. Science 346, 987991 (2014). Windows were grouped according to the number of hSNPs they contained. For each AugustusCGP transcript, if it had a better match to the CDS of any of the assigned transcripts than the current consensus transcript, the latter was replaced. Injected embryos were briefly cultured and oviductal embryo transfer performed in 0.5 days postcoital pseudopregnant female recipients (CBA/C57BL/6J). When mixing strains, consider the characteristics of each strain (e.g., Indica, Sativa, THC/CBD levels) and how they may complement or enhance each other. 9, e1003242 (2013). 102, 23692378 (2007). IRG proteins belong to a subfamily of interferon-inducible GTPases present in most vertebrates55. Fuller ME, Heraty L, Condee CW, Vainberg S, Sturchio NC, Bhlke JK, Hatzinger PB. Many hSNP clusters overlap immunity (for example, MHC, NOD-like receptors, and AIM-like receptors), sensory (for example, olfactory and taste receptors), reproductive (for example, pregnancy-specific glycoproteins and sperm-associated E-rich proteins), and neuronal- and behavior-related genes (for example, itch receptors26 and -protocadherins27) (Fig. We used the C57BL/6J GRCm38 sequence as a single reference and found that 95% adjacent synteny block pairs from the assemblies were also adjacent in C57BL/6J reference. These coordinates were then used to estimate the combined density of hSNPs using a 10 kb sliding window (step of 2 kb) across the mouse reference genome. AugustusPB transcripts that could not be confidently assigned to parent transcripts were discarded and not evaluated for novel contribution. Here we generate the first chromosome-scale genome assemblies for 12 classical and 4 wild-derived inbred strains, thus revealing at unprecedented resolution the striking strain-specific allelic diversity that encompasses 0.52.8% (14.475.5 Mb, excluding C57BL/6NJ) of the mouse genome. was supported by a research grant from CONICYT/FONDECYT/REGULAR No.1171004 and the European Commission (EUFP7 BLUEPRINT grant HEALTH-F5-2011-282510). Disclaimer. Headspace 28g This represented the density at which the interval content and total unique overlapping base pairs was observed to be clustered around distinct loci (Supplementary Fig. sharing sensitive information, make sure youre on a federal We're still gathering information about the Headspace strain. For the latter, CDS and intron hints were generated from the GENCODE VM8 protein-coding gene set for the reference strain. 5). Mixing strains is best done with caution and a clear understanding of how each strain may affect you. 7 and 8). The assemblies also showed extensive diversity at each of the other loci examined: immunity-related GTPases (IRGs) and Schlafen family (Slfn). Trainwreck Rev. Best Cannabis Strains for Sexual Arousal If the AugustusCGP transcript introduced new intron junctions supported by RNA-Seq, then it was incorporated as a new isoform of that gene. 4) that appear to have arisen either through retrotransposition (~80%) or gene duplication events (~20%). It can give off an earthy, lemony, pungent, spicy, or dank aroma. In most cases where a new locus was predicted on the reference genome, we identified pre-existing, but often incomplete, annotation. 295, 112 (1999). Parameters for assembly are listed in Supplementary Table 18. Physiol. All AugustusCGP transcripts that partially aligned to a reference transcript annotated as pseudogene or non-coding gene were also discarded. 7). Nat. Anthrax lethal factor cleavage of Nlrp1 is required for activation of the inflammasome. Efcab3/Efcab13 encode calcium-binding proteins and the new gene primarily consists of repeated EF-hand protein domains (Supplementary Fig. Anthony DiMeo. Strain Efacb3-like is conserved in orangutan but reversed in gorilla and appears to have split into two separate protein-coding genes, EFCAB3 and EFCAB13, in the Homininae lineage. Google Scholar. Nat Genet 50, 15741583 (2018). These divergent profiles suggest that Nlrp1c is not the sole mediator of anthrax lethal toxin resistance in the mouse but several other members may be involved. Aldi Essential Oils, Harps Bar And Grill West Haven Ct, Whiteland Community High School Band Directors, Steamhouse Lounge Oysterfest 2022, Articles H